The Association of PLA2G7 Gene Polymorphisms with Serum Lp-PLA2 Activity and Lipid Profile in Han Chinese Patients with Coronary Heart Disease

Yanhai Wang,1 Yupeng Shi,2 Zhongwei Wu,1 Jiangfeng Gao,3 Jing Wang,3 Lei Li,4 Yugang Wan,4 MuGu Lang A,1 Jianwen Zhang,1 Hongbo Wang,3 Yu Hou3,5 1Department of Clinical Laboratory, Hohhot First Hospital, Hohhot, 010030, People’s Republic of China; 2Zhejiang Digena Diagnosis Technology CO., LTD, Zhejiang, 310030, People’s Republic of China; 3Cardiology Department, Hohhot First Hospital, Hohhot, 010030, People’s Republic of China; 4Network Management, Hohhot First Hospital, Hohhot, 010030, People’s Republic of China; 5Cardiology Department, Inner Mongolia People’s Hospital, Hohhot, 010017, People’s Republic of ChinaCorrespondence: Yu Hou, Cardiology Department, Inner Mongolia People’s Hospital, No. 26, Zhaoda Road, Saihan District, Hohhot, Inner Mongolia Autonomous Region, 010017, People’s Republic of China, Tel +86-471-5281258, Email [email protected]: This study aimed to investigate the distribution patterns of PLA2G7 gene variants in Han Chinese patients with coronary heart disease (CHD), and their relationships with serum lipoprotein-associated phospholipase A2 (Lp-PLA2) levels and lipid profiles.Methods: A total of 93 han Chinese CHD patients were recruited. Serum Lp-PLA2 levels were determined using enzyme-linked immunosorbent assay (ELISA), while comprehensive analysis of PLA2G7 gene polymorphisms was conducted through whole-exome sequencing. Concurrently, multiple lipid parameters were measured and analyzed.Results: Among these Han Chinese CHD patients, the PLA2G7 gene rs1051931 (c.1136T>C p.Val379Ala) rare variant was highly prevalent (variant rate: 94.62%) among the study population, and showed negative correlation with serum Lp-PLA2 activity. The rs1765208290 (c.233G>A p.Gly78Asp) rare variant showed positive correlation with TG, ApoA, ApoB, HDL, LDL and TCHO levels in the serum. Strong linkage disequilibrium was observed between the rs1805018 (c.593T>C p.Ile198Thr) and rs76863441 (c.835G>T p.Val279Phe), both of which were related to lower Lp-PLA2 activity.Conclusion: In these Han Chinese CHD patients, the rs1051931 (c.1136T>C p.Val379Ala) rare variant in the PLA2G7 gene is closely linked to decreased Lp-PLA2 activity, whereas the rs1765208290 (c.233G>A p.Gly78Asp) rare variant influences lipid homeostasis. The strong LD between rs1805018 (c.593T>C p.Ile198Thr) and rs76863441 (c.835G>T p.Val279Phe) loci may act synergistically to reduce Lp-PLA2 activity.Keywords: PLA2G7 gene, coronary artery atherosclerotic heart disease, serum Lp-PLA2, lipid profile