The Association Between the rs2200733 SNP and Atrial Fibrillation Among Arabs: A Study from Jordan

Abdullah H Al-Kasasbeh,1 Omar F Khabour,2 Rowida Almomani,2 Muhannad Ababneh,1 Rashid Ibdah,1 Mohamad Ismail Jarrah,1 Sukaina I Rawashdeh,1 Abdelsamea Mohammed Seif1 1Department of Internal Medicine, Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan; 2Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, Jordan University of Science and Technology, Irbid, JordanCorrespondence: Omar F Khabour, Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, Jordan University of Science and Technology, P.O.Box 3030, Irbid, 22110, Jordan, Tel +962 790453765, Email [email protected]: Atrial fibrillation (AFib) is a common disorder featured by an irregular and fast heartbeat. The etiology of AFib is complex and involves genetic and environmental factors. The rs2200733 single nucleotide polymorphism (SNP) is located in close proximity to the promoter of paired-like homeodomain transcription factor 2 (PITX2) which plays a role in heart development.Objective: In this study, the association between the rs2200733 SNP and AFib was examined in the Jordanian population.Methods: The study included 450 subjects (274 controls and 176 patients with AFib). Patients were recruited from King Abdullah University Hospital based on the European Society of Cardiology criteria. The rs2200733 SNP was genotyped using restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) technique.Results: The mutant T allele of the rs2200733 SNP was common in the studied population with a frequency of 19%. The T allele and CT/TT genotypes were prevalent among patients with AFib compared with the controls (P< 0.05, OR [CI]: 1.65 [1.12– 2.43]). In addition, body mass index, diabetes, and hypertension were found to be associated with AFib risk.Conclusion: The rs2200733 SNP was associated with AFib among Jordanian patients. The mutant T allele of the rs2200733 SNP might increase the risk of AFib.Keywords: atrial fibrillation, rs2200733, PITX2, RFLP, Jordan, Arabs