Tissue Culture of Sainfoin

The effecls of 1-napthaleneacetic acid (NAA) and 6-benzylamino purine (BAP) on callus induction, callus proliferation and plant regeneration were examined ip sainfojn (011ob1ycl1is viciifo/ia Scop.) from differen! ex plant. Then the effects of indole-3-butyric acid (IBA) on root formation from differentiated shoot from calli were examined. The combinations of 1-10 µM ofNAA an4 BAP were better for sainfoin culture. For callus induction, there was no significant difference among 3 explants, but Chere were interactions between explant and media. For plant regeneration, hypocotyl and root are ��ter explants than cotyledon. The effects of NAA and BAP showed less effective on callus induction and proliferation, but much better on shoot for��ation °than tho��e of 2,4- D and BAP. IBA plays an important role for root formation. The best concentration of IBA was 2S µM.