Extraction and Chromatographic Separation of Antimicrobials Produced by a Strain of Burkholderia

“Burk” is the working name of a bacteria collected by a JCCC microbiology student previously been shown to inhibit tester strains of bacteria when grown competitively. Chemical extracts taken from these bacteria inhibit tester strains of both E. coli and S. epi when grown in broth with the extract. This research explored the separation and isolation of components of the extract mixture using chromatography. The separated fractions were tested for antimicrobial activity against tester strains in broth. The results will be discussed.