Characterization of Peripheral Nerve Demyelination Model

Demyelination disorders impair nerve signal conduction by reducing myelin, particularly at the nodes of Ranvier, leading to motor deficits. To model peripheral nerve demyelination, we performed partial ligation of the sciatic nerve in C57BL/6J mice, monitoring them via immunohistochemistry, TEM imaging, and behavioral tests over 14 days. We observed decreased myelin basic protein, reduced neurofilament levels, and thinner myelin layers. Behavioral deficits emerged as early as day 3. These findings establish a reproducible model correlating molecular, structural, and functional deficits, paving the way for targeted therapeutic strategies aimed at restoring nerve conduction in demyelinating disorders.