Antibiotics in Soil

In this experiment we studied the soil samples that were taken from a designated spot in order to determine if there are antibiotics within the sample. By completing serial dilution of the sample, creating a master plate of the bacteria, testing the bacteria with the ESCAPE relatives to determine what will inhibit other known bacteria. We followed that up by staining the bacteria using endospore staining to identify bacteria present, sequencing the results, and completing metabolic tests to identify what bacteria will produce antibiotics.