The Search for Antimicrobials within Soil

The overall goal of this research project is to discover antimicrobial species from a soil sample. A soil sample was obtained and diluted using the process of serial dilution. Candidates with inhibition were collected and cultured from the serial dilution plates to form a master plate. The candidates acquired from the master plate were then tested against safe relatives of ESKAPE pathogens to determine if they will prevent growth against those pathogen strains. Metabolic testing, PCR, and gel electrophoresis were used to determine the identity of the candidate.