Investigating the Expression of Vascular Endothelial Growth Factor (VEGF) Gene Related to Angiogenesis and Interleukin-10 in Experimental Breast Tumor Model

Introduction: Breast cancer is one of the most common cancers and the principal cause of death in women. One of the mechanisms of cancer cells for the lack of access to the immune system is the production of compounds suppressing immune responses, such as interleukin-10. On the other hand, vascular endothelial growth factor, by binding to its receptors on the surface of endothelial cells, plays a significant role in vascular permeability and tumor vascularity. In this study, the expression of interleukin-10 and vascular endothelial growth factor in breast tumor tissue was investigated in an experimental tumor model. Methods: First, mammary tumors were experimentally induced in Balb/C mice, and RNA was extracted from the tumor tissue. cDNA was synthesized from the extracted RNAs, and the expression level of 10-IL and VEGF genes was evaluated by RT-PCR. Results: The results of data analysis showed that the expression of IL-10 and VEGF genes in the tumor tissue was higher than in the cells of the control group, but this increase in expression was not statistically significant. Conclusions: In general, the expression level of Interleukin-10 and VEGF genes was increased in the experimental tumor model, but broader research and the correlation with other involved factors seem necessary.