Enzymatic oligomerization of phloridzin in betaine-based deep eutectic solvent

Introduction: Laccase-catalyzed oligomerization of flavonoids has been investigated in many studies where the requirement of certain amount of organic solvent to provide the solubility of the monomer and products has been noted. Recently, the usage of more environmentally friendly medium has attracted significant attention. Deep eutectic solvents (DES) are a new type of solvents which have gained much attention and have been tested for many applications, including biocatalysis. As they are considered as green solvents, their combined use with laccases can enhance the greenness of the oligomerization process. In this work, DES betaine:sorbitol:water (B-S) was used in different concentrations as co-solvents in oligomerization of flavonoid phloridzin catalyzed by laccase from Trametes versicolor. ...