Development of a Miniaturized Extracorporeal Membrane Oxygenation (ECMO) Device on a Microfluidic Platform

This research investigates the design, performance, and optimization of blood-contacting microfluidic medical devices, with each aim contributing to a broader understanding of how engineering choices influence hemocompatibility and oxygen transfer. Collectively, the findings from Aims 1A–2B offer integrated strategies to develop safer, more effective microfluidic systems for ECMO and dialysis. Aim 1A assessed hemolysis across device geometries using computational and experimental methods. All designs showed low device-induced hemolysis (< 2 ppm), validating their hemocompatibility. However, Power-Law models frequently overpredicted hemolysis, indicating a need for refined parameters tailored to microfluidic flow conditions. Aim 1B demonstrated the effectiveness of thrombosis assay techniques in evaluating clot formation in microfluidic device geometries. Results showed that geometry and local shear patterns strongly influence thrombogenesis. While hemolysis remained minimal even in high-shear devices, thrombus formation was more geometry-sensitive—underscoring the need to account for both factors in design. Modifying surface properties and accounting for blood composition could further reduce thrombotic risk. In Aim 2A, we compared PDMS, polypropylene (PP), and nanoporous silicon nitride (NPSiN) membranes for oxygen transport. Findings showed that in ECMO devices, blood-side resistance, not membrane permeability, often dominates oxygen transfer. Thus, membrane choice must be paired with optimized blood-side flow for full performance gains. Aim 2B directly addressed these limitations by integrating staggered herringbone mixers (SHBs), which introduced secondary flows and enhanced mixing. The Single Channel Herringbone design yielded the highest oxygen transfer, confirming the synergy between flow geometry and oxygen transport performance. Altogether, Aims 1A and 1B establish a strong framework for hemocompatibility evaluation, while Aims 2A and 2B provide complementary insights into optimizing oxygen transfer. Their interconnections highlight the value of a systems-level approach to designing microfluidic blood-contacting devices.