Deneysel Sıçan Kolit Modelinde Alfa Lipoik Asit Tedavisinin SOD ve NLRP3 Seviyesi Üzerine Etkisi

Ulcerative colitis is a disease accompanied by chronic inflammation of the mucosa and submucosa layers of the colon. Colon inflammation causes the release of reactive oxygen and nitrogen derivatives due to excessive activation of immune system cells, and oxidative stress. In our study, the serum and colon NOD-like receptor protein 3 (NLRP3) levels and colon superoxide dismutase (SOD) levels were investigated in the acetic acid-induced colitis model in rats. Furthermore, this study aims to investigate the regulatory effects of alpha-lipoic acid, a potent antioxidant, on these parameters. In our study, 34 healthy 8-10 week-old male Wistar Albino rats weighing 200-250 g were used. The animals were divided into 5 groups with 6 in the control group and 7 in each study group. To induce colitis, 4% acetic acid was administered intrarectally to the animals, except for the control group. Subsequently, treatment was initiated with alpha-lipoic acid, mesalamine, and a combination of both, continuing for 14 days. Diarrhea scores of the animals were recorded throughout the treatment procedure. After the experiment, serum and colon tissue NLRP3, as well as SOD levels in the colon were evaluated. The diarrhea score was observed to significantly increase in the colitis group compared to the other groups, while it significantly decreased in the mesalamine+alpha-lipoic acid group. In the colitis group, NLRP3 levels in the serum and colon tissue were the highest, while colon SOD levels were the lowest. These parameters were significantly regulated in the mesalamine+alpha lipoic acid group compared to the other groups. In conclusion, it was determined that NLRP3 inflammasome activity increases and SOD levels decrease in cases of colitis; moreover, the combination of alpha-lipoic acid with mesalamine had a more pronounced regulatory effect on these values compared to their use alone.