ЕКСПЕРИМЕНТАЛЬНЕ ОБҐРУНТУВАННЯ МЕТОДУ ОЦІНЮВАННЯ ПОШИРЕНОСТІ ГОСТРОГО ПЕРИТОНІТУ

Abstract Objective. To explore way for objectively assessing the spread of acute peritonitis in experimental settings. Materials and methods. A study was conducted on 50 albino non-pedigree rats, divided into two groups: 10 served as the control group, and 40 were part of the research group. Acute peritonitis was induced in the research group through an intraperitoneal injection of a 20 % autofecal mixture. Both groups underwent laparotomy at intervals of 6, 12, 24, and 48 hours following the induction of peritonitis. During these procedures, the width of the scattering zone of laser beams with wavelengths of 0.63 μm and 0.4 μm was measured on the parietal peritoneum. Subsequently, the peritoneum was collected for histological examination. Results. In the initial stages, no changes were observed in the histological structure of the peritoneum and adjacent tissues. However, serous inflammation of the peritoneum was detected 6 hours after modeling, which later progressed to purulent inflammation. At the same time, the scattering zone of a laser beam with a wavelength of λ=0.63 μm increased significantly and continued to do so consistently. A similar pattern was observed for a beam with a wavelength of λ=0.4 μm. By 48 hours, the measurements were similar to those at 24 hours. The ratio of the scattering zone widths for the two wavelengths increased significantly 6 hours after simulating peritonitis and remained stable thereafter. Conclusions. 1. The scattering zone width ratio of laser beams with wavelengths of λ=0.63 μm and λ=0.5 μm shows a statistically significant difference between intact and inflamed areas of the rats' parietal peritoneum. 2. In the intact peritoneum, the ratio indicators are less than 2 units, whereas in the inflamed peritoneum, these parameters increase significantly.