Study of ASF1a Protein’s Biological Significance in Arabidopsis thaliana via Phenotypic Manifestations of CRISPR/CAS Induced A. thaliana ASF1a Mutagenesis

ANTI-SILENCING FUNCTION 1A (ASF1a) is a multifunctional protein involved in pivotal cellular processes. In plants, ASF1a interacts with HIRA to participate in KNOX genes silencing. To further characterize how ASF1a regulates gene expression, a CRISPR CAS 9 vector was created and ready to be transferred into Arabidopsis thaliana to induce mutations in the 3’ region of the A. thaliana ASF1a gene that corresponds to the ASF histone chaperone protein domain. We hypothesized that phyllotactic alterations will take place as a result of the mutations.