Optimization of the Extraction of Polyphenolic Compounds from Oil palm Bunches
\nand their Potential Application in Fish Feed

Oil palm bunch is one of the biggest agroindustry wastes in Indonesia. Polyphenolic compounds are compounds
\nthat could be found in many plants and obtained through extraction, including extraction of oil palm bunch.
\nPolyphenolic compounds have various benefits, including antioxidant properties. This study aims to determine the
\nsolvent concentration and the solvent to mass ratio in the extraction of polyphenols from oil palm bunches and
\nthen formulate it in fish feed to study the resulting effects. From this research, we found that 50% methanol solvent
\nis the most optimal for oil palm bunches extraction with Total Phenolic Content (TPC) 1.56±0.125 mg Gallic Acid
\nEquivalent (GAE) / g sample, antioxidant activity 3.08±0.08 μmol GAE / g sample (DPPH), and antioxidant
\nactivity 3.13±0.96 μmol GAE / g sample (FRAP). We also found that 40:1 is the most optimal extraction mass
\nratio with TPC 1.62±0.07 mg GAE / g sample, antioxidant activity 5.07±0.09 μmol GAE / g sample (DPPH), and
\nantioxidant activity 3.82±0.35 μmol GAE / g sample (FRAP). When used in fish feed, polyphenols do not affect
\nthe length, growth, and mass of molly fish under stress conditions.