Extraction, Separation, and Testing of Antimicrobial Compounds Produced by a Strain of Burkholderia Bacteria

A bacteria collected by a JCCC microbiology student called, “Burk,” has previously been shown to inhibit S. epi when the two bacteria are grown in competition. Chemical extracts taken from these bacteria inhibit both E. coli and S. epi when grown in broth with the extract. This research explored the separation and isolation of components of the extract mixture using chromatography. The separated fractions of the extract were tested for antimicrobial activity against tester strains in broth. The results will be discussed.