Exploring Host Range of Klebsiella pneumonia

Bacteriophages, or phages, are viruses that target bacteria. Lytic phages cause the bacteria to lyse (cell death). Klebsiella pneumoniae is a bacterium that is associated with severe urinary tract infections. Some K. pneumoniae are hypervirulent—causing difficult-to-treat infections. Lytic phages of K. pneumoniae can functionally limit bacteria populations. This project explores this dynamic. Strains of K. pneumoniae were mixed with soft agar and poured onto LB plates. The viruses were then spotted in the soft agar cultures. Lysis of bacteria causes clearings after virus introduction. Successful phages could be used in a clinical setting as infection treatment.