Développement et applications de nouvelles sondes fluorescentes

Fluorescent probes are essential tool for studying biomolecular interactions due to their high sensitivity and adaptability. This thesis is based on epicocconone, a pro-fluorescent compound, known for its distinctive spectral characteristics, to develop new analogues with enhanced fluorescence and broadened functionality, specifically targeting applications in studying the immunosuppressant drug FK506. The project consists of three main aims: 1) It focuses on improving previously synthesized epicocconone analogues by substituting the keto-enol moiety, which contributes to non-radiative de-excitation wavelength, with a conjugated heteroaromatic rings. 2) Inspired by a natively fluorescent epicocconone-hemicyanin hybrid developed in our lab, we aim to create new analogues featuring a pyrazole ring for native fluorescence. This advancement will support 3) the synthesis of a fluorescent FK506 probe, which will enable FK506 binding studies using a yeast display system.