Biological Test Methods – Model for Assessing the Neutralization of Sewage Sludge Pollutants Using β-Diketonate

The subject of this study is the application of an innovative approach to the neutralization of pollutants from sewage sludge taken from the Cvetojevac wastewater treatment plant in Kragujevac (Republic of Serbia). The aim of the research was to investigate the possibility of using β-diketonate as a means of neutralizing pollutants from sewage sludge using a modified bioassay method (sandwich method). Red clover (Trifolium pratense L.) was used as a model organism to test seed germination in sludge treated with synthesized β-diketonate at varying concentrations (10, 30, 50, 100, 200 mg/L). The results indicate that β-diketonate application significantly affected the germination rate and root growth of T. pratense. The synthesized β-diketonate demonstrates potential for pollutant neutralization in sewage sludge and could be applicable in horticulture, agriculture, and environmental remediation