Study on hsa_circ_101209 in Plasma of Pregnant Women with Deep Venous Thrombosis

Yuhong Zhang, Jun Li Center for Obstetrics and Reproductive Medicine, The Affiliated Hospital of Yunnan University, Kunming City, Yunnan Province, 650032, People’s Republic of ChinaCorrespondence: Jun Li, Center for Obstetrics and Reproductive Medicine, The Affiliated Hospital of Yunnan University, No. 176, Qingnian Road, Kunming, Yunnan, 650032, People’s Republic of China, Email [email protected]: This study analyzed the expression and diagnostic value of hsa_circ_101209 in plasma of pregnant women with in deep vein thrombosis (DVT).Methods: By circRNA microarray detection and GO/KEGG analysis, hsa_circ_14797 targeting miRNA-mRNA network was predicted. Sixty women with DVT were selected as the DVT group, and 60 women without DVT as the non-DVT group. hsa_circ_14797 in plasma was detected, as well as D-dimer (D-D) concentration and P-selectin expression. Target genes that may be regulated by hsa_circ_14797 were predicted, and GO analysis and KEGG pathway enrichment analysis were performed.Results: hsa_circ_14797 was highly expressed in DVT. hsa_circ_14797 in plasma of DVT patients was positively correlated with D-D (r = 0.358, P = 0.001). The AUC of plasma hsa_circ_14797, D-D, and P-selectin for maternal DVT diagnosis were 0.787 (95% CI: 0.710– 0.864), 0.882 (95% CI: 0.821– 0.943), and 0.825 (95% CI: 0.754– 0.895), respectively. The AUC of hsa_circ_14797 combined with D-D was 0.886 (95% CI: 0.828– 0.944). The AUC of hsa_circ_14797 combined with P-selectin was 0.904 (95% CI: 0.853– 0.954). The AUC of P-selectin combined with D-D was 0.935 (95% CI: 0.893– 0.978). The AUC of hsa_circ_14797 combined with D-D and P-selectin was 0.953 (95% CI: 0.920– 0.986). The functions of hsa_circ_14797 included the biological processes of angiogenesis, vascular development, and vascular morphology. The enrichment pathways included PI3K-Akt pathway, TGF-β pathway, and cytokine-cytokine receptor interaction.Conclusion: hsa_circ_14797, D-D, and P-selectin in plasma of DVT pregnant patients are increased, and hsa_circ_14797 in plasma is positively correlated with D-D. hsa_circ_14797 combined with D-D and P-selectin can improve the accuracy of diagnosis of DVT and contribute to the early diagnosis of DVT.Keywords: Deep vein thrombosis, circRNA, D-dimer, P-selectin, Biomarkers