Searching for and Identifying Antibiotic Producing Bacteria From Soil

Antibiotic resistant bacteria pose a growing health crisis, as they outpace the development of new antibiotics. Tiny Earth is an initiative to combat this growing crisis by systematically screening soil samples for new antibiotic producing organisms by sourcing the work of students. Serial dilutions are one of the major steps in isolating antibiotic producing bacteria. Here we report a successful serial dilution, stains, PCR and gel electrophoresis. Candidate 4 had a large area of inhibition around S.epi and a smaller area of inhibition around E.coli.