Optimización de protocolo de establecimiento in vitro de Magnolia arcabucoana (Lozano) Govaerts. (Magnoliaceae) como una herramienta de conservación

Contextualization: Magnolia arcabucoana (Lozano) Govaerts, is a species categorized as endangered, with deficient sexual propagation due to low fruit and seed production, which generates a high vulnerability of existing populations to extinction events. In vitro tissue culture techniques are an effective alternative for the propagation of threat species that require specific strategies for their conservation. Knowledge gap: At present, there is no protocol that creates optimal conditions to enable the disinfection and in vitro establishment of Magnolia arcabucoana. Purpose: Establish and optimize a protocol for the disinfection and in vitro establishment of vegetative buds as a conservation tool for the species. Methodology: Three experimental trials were conducted corresponding to a disinfection protocol, phenol inhibitor agents, and establishment. DCA experimental designs and existing methodologies for each protocol were employed. Results and conclusions: It was obtained that concentrations of 1 g L-1 of CA (Activated Carbon) + 0.5 g L-1 of AA (Ascorbic Acid) included in the culture medium and AA solution at 25 % w/v for 15 minutes, 67.7 % controlled phenolic oxidation and bud necrosis. The best disinfection treatment was achieved with inversion for three minutes in a concentration of 0.8 % of Ca(ClO)2. In the establishment phase, the greatest growth of the buds was achieved with Woody Plant Medium (WPM) with 1 mg L-1 of BAP (6 Benzyl Aminopurine) + 0.05 mg L-1 of ANA (Naphthaleneacetic acid). Sprouting was obtained 105 days after establishment in the WPM medium with 3 mg L-1 of BAP. The results constitute a contribution for the in vitro establishment of Magnolia arcabucoana for conservation purposes