Identificación metagenómica de microorganismos del líquido ruminal y humus de lombriz, biodegradadores de plásticos

Biodegradation is a method widely used in the bioremediation of polymers that allows controlling the alteration of ecosystems damaged by anthropogenic activities. The objective was to determine the biodegradation capacity of the microbial consortium of bovine ruminal fluid and worm humus on low-density polyethylene and terephthalate at different temperature and humidity conditions, and to identify the microorganisms involved in this process. The methodology was experimental in which 120 sheets of PET and 120 of PEBD measuring 2.5 cm x 2.5 cm were used, submerged in sterile jars with ruminal fluid and humus. The sheets and their repetitions were removed from the jars monthly. for 7 months. The identification of the microorganisms was developed through a metagenomic analysis, which allowed the identification of microorganisms in the ruminal fluid and in the worm humus, finding the most common genera Bacillus, Methanosarcina and Advenella, in addition the highest biodegradation value was 4.5% for the treatment of worm castings with low-density polyethylene at an incubation temperature between 25° to 27°C.