The efficacy of bioproduct of Bacillus spp. on mycelium growth and controlling green mold disease of Ganoderma lucidum

This research aimed to study the potential of bioproduct of Bacillus spp. on promoting mycelial growth and controlling green mold disease caused by Trichoderma velutinum in Ganoderma lucidum. The antagonistic native Bacillus three isolates via Bacillus subtilis subsp. subtilis RUTs001, Bacillus subtilis RUTs002 and Bacillus subtilis RUTs003 were used to test interaction with G. lucidum mushroom mycelium and control green mold disease in the laboratory by dual culture and in a mushroom house. The results showed that all Bacillus spp. had a positive interaction with G. lucidum on PDA plates, with mushroom mycelium growth of 79.29 - 100.00%. Additionally, there were no harmful effects found on the mycelium of G. lucidum. The growth inhibition of T. velutinum by Bacillus spp. was 22.28 - 73.93%. Biological products of Bacillus spp. were significantly different in their effectiveness in controlling green mold disease in mushroom houses (P<0.01). The combination of B. subtilis subsp. subtilis RUTs001, B. subtilis RUTs002 and B. subtilis RUTs003 showed the lowest disease incidence and disease severity index of 12.50 and 2.08%, respectively. Yield was 158.24 g/bag and biological efficiency (B.E.) was 40.26%, There was no significant difference form control checked (normal spawn) disease incidence, disease severity index, yield and biological efficiency obtained 4.17%, 0.69%, 162.96 g/bag and 41.47%, respectively.