Dilution Revolution

The overall goal of this research project was to identify and characterize potential antimicrobial producing microorganisms. Through a serial dilution of a soil sample collected and cultured to isolate bacterial candidates created on a master plate isolating bacteria. Later using an antibiotic screening on different bacteria to screen for zones of inhibition where bacteria did not grow. Metabolic testing was performed to analyze biochemical properties, while PCR and gel electrophoresis were used to determine the genetic identity if candidates found in our antibiotic screenings. Helping confirm whether the isolated bacteria could produce antimicrobial compounds for the search of new antibiotics.