Antimicrobial and Cytotoxic Effect of Bornetella Nitida Green Algae Isolate on MCF-7 Breast Cancer

Yuni Elsa Hadisaputri,1 Nunuk Hariani Soekamto,2 Pratiwi Pudjiastuti,3 Aria Aristokrat,1 Bahrun Bahrun,2,4 Fajar Fauzi Abdullah,5 Tatsufumi Okino4 1Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor, West Java, 45363, Indonesia; 2Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Makassar, Indonesia; 3Department of Chemistry, Faculty of Mathematics and Natural Sciences, Airlangga University, Surabaya, Indonesia; 4Faculty of Environmental Earth Science, Hokkaido University, Hokkaido, Japan; 5Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Garut, Kabupaten Garut, IndonesiaCorrespondence: Yuni Elsa Hadisaputri, Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang KM.21, Jatinangor, West Java, 45363, Indonesia, Tel +62-22-84288888, Email [email protected]: Marine algae are increasingly becoming a potential resource for new drugs. In recent decades, including Bornetella nitida (B. nitida). Meanwhile, antimicrobial and anticancer agents are the first line of choice for developing alternative compounds, considering the annually increasing resistant events. Therefore, this study aimed to examine the antimicrobial and cytotoxic potential of B. nitida isolate compounds.Methods: The B. nitida resulted in 2 compounds, sitosterol 3β tetracosanoate and (E)-17-(8-ethyl-4,5,9-trimethyldec-6-en-2-yl)-13-methyl-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-3-ol. Both compounds were tested to have antibacterial effects against Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus, Methicillin-resistant Staphylococcus Aureus (MRSA). Proliferation assay was conducted using the PrestoBlue™ Cell Viability Reagent, which was also used to measure the IC50 against MCF-7 breast cancer cells.Results: The results showed that (E)-17-(8-ethyl-4,5,9-trimethyldec-6-en-2-yl)-13-methyl-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-3-ol had antimicrobial activity against Staphylococcus aureus and IC50 value of 142.18 μg/mL against MCF-7 cells, while sitosterol 3β tetracosanoate does not have any antimicrobial activity and IC50 value of 681.65 μg/mL. Moreover, the mechanism prediction using docking with caspase-3 receptor to induce apoptosis was also evaluated.Conclusion: Based on the results, (E)-17-(8-ethyl-4,5,9-trimethyldec-6-en-2-yl)-13-methyl-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-3-ol of B. nitida has great potential as an antimicrobial and anticancer agent.Keywords: Bornetella nitida, Staphylococcus aureus, MCF-7, cytotoxic, apoptosis