Розробка технології отримання сухого екстракту «Гельмінт АРТ» та дослідження його протигельмінної активності

The aim. This research work is devoted to the development of an optimal method for obtaining anthelmintic dry extract "Helmint-ART" from raw materials of plants growing in Uzbekistan: aerial (aboveground) part of wormwood, pumpkin seeds, tansy flowers, garlic bulbs and to study the acute toxicity and anthelmintic activity. Materials and methods. Two different methods for obtaining dry extract (DE) "Helmint-ART" from plant raw materials: the aerial part of wormwood, pumpkin seeds, tansy flowers and garlic bulbs were used. The first method: prepare an extract of plants raw materials and then prepare DE substance from them. The second method: a separate DE was obtained from each selected object and then mixed in a certain ratio. Quantitative indicators of biologically active substances were determined with high-performance liquid chromatography. The study of anthelmintic effect of the DE "Helmint-ART" was carried out on intestinal parasitosis experimental models in mice: hymenolepidosis (causative agent Hymenolepis nana) and aspiculurosis (causative agent Aspiculuris tetraptera). Results. It was found that the second method for obtaining DE named "Helminth-ART" was optimal. Determination the "acute" toxicity showed that DE "Helminth-ART" at a dose 3000 mg/kg do not cause changes in animal’s behavior and their death. Anti-cestode activity of DE "Helmint-ART" was 74.9 % and was slightly higher (statistically unreliable) than that of comparison drug Fenasal (71.2 %) and significantly exceeded that of the infusions of common tansy flowers (50.2 %), pumpkin seeds (56.8 %). Anti-nematodosis activity (90.2 %) of DE "Helminth-ART" significantly exceeded the activity of infusions of tansy flowers (68.6 %), pumpkin seeds (76.9 %), Pyrantel (80.1 %). Conclusions. The optimal method of the technology of the DE "Gelmint-ART" was determined based on a comparison of two methods. The second method is optimal in terms of economy of extractants, high proportion of the DE released, their quality indicators, content of the biologically active substance luteolin. The pronounced DE "Helmint-ART" anthelmintic effect and acceptable acute toxicity were established