The Combination of Super-Active Platelet Lysate and Acellular Amniotic Membrane Enhances Endometrial Receptivity, While Simultaneously Facilitating Endometrial Repair in Rats

Huaying Wu,1 Yi Zhang,2 Chunxiang Liu,2 Xiaohan Tang,1 Liqun Wang,1 Lingqi Meng,2 Meisong Lu1 1Department of Obstetrics and Gynecology, The First Affiliated Hospital of Harbin Medical University, Harbin, People’s Republic of China; 2National and Local Joint Stem Cell Research & Engineering Center for Aging Diseases, Tian Qing Stem Cell Co., Ltd, Harbin, People’s Republic of ChinaCorrespondence: Meisong Lu, Email [email protected]: To investigate the combined effects of super-active platelet lysate (sPL) and acellular amniotic membrane (AAM) in promoting endometrial repair and enhancing endometrial receptivity in rats.Methods: The characteristics of sPL-AAM were examined through scanning electron microscopy, contact angle tester, and release experiments. We aimed to establish a rat model for endometrial injury. We divided sixty-four rats into four groups: the Injury group (Control group), the AAM group, the sPL group, and the sPL-AAM group. Our study compared the endometrial thickness, gland count, and fibrotic area recovery in rats at 6 days and 18 days post-treatment. Immunohistochemistry was utilized to assess the expressions of CD34 and ANG. Additionally, we used ELISA to detect the levels of IL-6 and TNF-α, while Western Blot was employed to compare the expressions of CK19, Integrin β 3, and TGF-β 1. One month after the treatment, we evaluated and compared the pregnancy recovery among the groups.Results: Compared to the Injury group, the sPL-AAM group demonstrated enhanced endometrial regeneration in rats at both 6 days and 18 days post-treatment, resulting in a favorable pregnancy outcome. This was achieved by promoting angiogenesis, suppressing the inflammatory response, and reducing fibrosis. The observed effects were superior to those of the sPL group alone. While sPL, when administered alone, showed some degree of endometrial restoration at 6 days post-treatment, its efficacy was diminished at 18 days post-treatment. The impact of AAM alone appeared inconspicuous compared to the injury group. This suggests that sPL serves as the primary agent in facilitating endometrial repair, while AAM functions as a carrier to extend the duration of sPL’s effectiveness.Conclusion: sPL-AAM can release effective cytokines, repair endometrial damage in rats, enhance endometrial receptivity, and ultimately improve pregnancy outcomes.Keywords: sPL, AAM, endometrial damage, endometrial regeneration