Chromatographic Separation of Extracted Antimicrobials Produced by a Pseudomonas Bacteria

A bacteria collected by a JCCC microbiology student called, “Betty,” has previously been shown to inhibit tester strains of bacteria when they are grown competitively. Chemical extracts taken from these bacteria inhibit tester strains of both E. coli and S. epi when grown in broth with the extract. This research explored the separation and isolation of components of the extract mixture using chromatography. The separated fractions of the extract were tested for antimicrobial activity against tester strains in broth. The results will be discussed.