Whole-Genome Analysis of Paenibacillus campinasensis from Blood of a Hand-Foot-Mouth Patient

Xiaoling Yu,1 Jun Lin,2 Wenqian Jiang,2 Tianzhu Zhu,2 Xiao Lin,3 Xiaolong Liu,4 Yongyi Zeng5 1Department of Pharmacy, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, People’s Republic of China; 2Institute of Applied Genomics, Fuzhou University, Fuzhou, People’s Republic of China; 3Department of Critical Care Medicine, the First Affiliated Hospital of Fujian Medical University, Fuzhou, People’s Republic of China; 4The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, People’s Republic of China; 5Department of Hepatobiliary Surgery, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, People’s Republic of ChinaCorrespondence: Xiaolong Liu, The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, No. 312 Xihong Road, Gulou District, Fuzhou, 350025, Fujian Province, Email [email protected] Yongyi Zeng, Department of Hepatobiliary Surgery, Mengchao Hepatobiliary Hospital of Fujian Medical University, No. 66 Jintang Road, Cangshan District, Fuzhou, 350001, Fujian Province, Email [email protected]: This study aims to investigate the antibiotic resistance and virulence factors of Paenibacillus campinasensis, an understudied opportunistic pathogen, through whole-genome sequencing and resistance genes. The findings will contribute to the development of effective clinical strategies for infection control.Patients and Methods: A 1-year and 3-month-old boy presenting with fever and rash was admitted to the hospital and clinically diagnosed with hand-foot-mouth disease. A blood culture was obtained, and the pathogen was preliminarily identified as belonging to the genus Paenibacillus using MALDI-TOF-MS. Subsequent 16S rDNA PCR product Sanger sequencing confirmed the isolate as Paenibacillus campinasensis. Whole-genome sequencing and bioinformatics analysis were performed to evaluate the antibiotic resistance and virulence factors of the strain.Results: Whole-genome sequencing and bioinformatics analysis identified 18 resistance genes in the Paenibacillus campinasensis strain, spanning multiple antibiotic categories. These findings suggest the potential for multidrug resistance in this bacterium, despite its isolation from blood without associated clinical infection in the patient.Conclusion: The identification of resistance genes in Paenibacillus campinasensis underscores the challenges in clinical treatment and emphasizes the critical role of whole-genome analysis in pathogen detection and characterization. These findings are vital for informing effective clinical strategies to address infections, particularly given the potential for antibiotic resistance to hinder treatment outcomes, even in the absence of direct disease causation by the bacterium.Keywords: antibiotic resistance, whole-genome sequencing, multidrug resistance, bioinformatics analysis, virulence factors