The impact of modification in metabolism: A focus on MDH1

The cytoplasmic enzyme, Malate Dehydrogenase MDH1, contributes to the cellular metabolism and generation of energy. MDH has to be tightly regulated to respond to cellular needs. One type of regulation mechanism is the post-translational modification (PTM) of enzymes, such as ADP-ribosylation. Two amino acids in MDH1 have been identified as ADP-ribosylated by mass spectrometry, but the role of this PTM is unknown. Using in vitro approaches, we are assessing how the absence of ADP-ribosylated amino acids impacts the enzyme’s activity. This work helps us gain a better understanding of the regulatory role of PTM on MDH1 and metabolism.