Extraction and Separation of Antimicrobial Compounds Produced by a Strain of Pseudomonas Bacteria

“Betty” is the working name of a bacteria collected by a JCCC microbiology student. It has previously been shown to inhibit tester strains of bacteria when grown competitively. Chemical extracts taken from these bacteria inhibit tester strains of both E. coli and S. epi when grown in broth with the extract. This research explored the separation and isolation of components of the extract mixture using chromatography. The separated fractions were tested for antimicrobial activity in broth. The results will be discussed.