Development of 3D Glioma Models Based on Cell Spheroids Embedded in Pectin- Collagen Matrix

Three-dimensional cell culture is a modern tool for cancer research. This cell-based model
\ncombines the simplicity and accessibility of two-dimensional systems with mimicking in vivo conditions
\nvia supporting cell- cell and cell-matrix interactions. This allows it to be used both for studying the
\nmechanisms of cancerogenesis and for preclinical testing anticancer therapeutics. In this research, we
\npropose a simple and detailed technique for the preparation of spheroids composed of human glioblastoma
\ncells embedded in a biopolymer matrix based on modified pectins supplemented with collagen I. The
\nprotocol also includes long-term cell monitoring with comprehensive analysis of cell viability, migration
\nand behavior in culture using conventional optical imaging, laser scanning microscopy and flow cytometry