Correlation analysis between physical characteristics and seed vigor of the endangered plant Abies beshanzuensis

Abstract [Objective] This study aims to explore the relationship between seed morphological and physical characteristics and seed vigor of Abies beshanzuensis, in order to provide a theoretical basis for further population renewal and protection of this endangered species, explaining its endangered mechanism, and studying its seed biology. [Methods] Using the endangered plant A . beshanzuensis as material, characteristic constants of the length, width, thickness, single grain weight, and RGB, etc. of the seeds of A . beshanzuensis were measured. Seed vigor was determined using TTC staining method, and their mathematical models were established. [Results] Significant differences were observed in R, B values, and single seed weight between vigorous and non-viable seeds of A . beshanzuensis. Single factor linear regression analysis showed that the correlation between seed vigor and single seed weight of A . beshanzuensis was the most significant, with R2=0.839. Two factor linear regression analysis showed that seed vigor had the most significant correlation with single seed weight and width, with R2=0.895. For multivariate linear regression analysis, R2 =0.978. However, no significant differences were observed in length, width, thickness, and G value between vigorous and non-viable seeds. [Conclusion] By using indicators such as R, B value, and single grain weight of A . beshanzuensis seeds, seeds with higher seed vigor can be screened. Multiple factor linear regression analysis predicts more accurately the seed vigor