Additional file 5 of Analysis of early childhood intestinal microbial dynamics in a continuous-flow bioreactor

Supplementary Material 5: Figure 4. A library of early childhood gut bacterial isolates. Bacterial strains from the fecal-derived chemostat community of NS0 or NS1 individuals were isolated by axenic culture using a variety of media formulations (Suppl. Table 2 ). 80 unique bacterial strains were archived, constituting a library of NS0 sample (Suppl. Table 14 ), and 118 unique bacterial strains were archived, constituting a library of NS1 sample (Suppl. Table 15). (a) Phylogenetic tree of all gut bacterial isolates (species-level) identified by 16S rRNA gene sequencing of NS0 and NS1 libraries. Colored squares indicate phylum. (b) The relative abundance distribution of each isolate species in the fecal samples of CHILD cohort infants at 12 months old is shown (n=842 samples). 16S rRNA gene sequencing dataset from CHILD cohort was obtained at the Sequence Read Archive of NCBI via accession number PRJNA657821.