GENETIC POLYMORPHISMS OF INTERLEUKINS IL-1A, IL-1B, AND IL-1RN IN PATIENTS WITH PERIODONTAL DISEASE AND DENTO-MAXILLARY ANOMALIES

Background: The study aims to investigate the interaction between genetic polymorphisms of interleukins IL-1A rs1800587, IL-1B rs1143634, and IL-1RN rs419598 and dento-maxillary anomalies (DMAn) in periodontal disease (PD). Methods: Conducted at the “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania, this pilot study involved two groups: patients with DMAn and PD, and patients with PD without DMAn. We examined IL-1A rs1800587, IL-1B rs1143634, and IL-1RN rs419598 polymorphisms using microfluidic SNP genotyping. Results: Statistical analysis of genotypes indicated the following: (1) IL-1A rs1800587: The C/C genotype also showed significant trends (p=0.057), being more frequently found in PD patients without DMAn (90% vs. 40%); (2) IL-1B rs1143634: Significant trends were found for the C/T genotype (p=0.057), which was more frequently found in patients with PD and DMAn (60% vs. 10%); (3) IL-1RN rs419598: The analysis did not show statistically significant differences between the two groups (p=0.332). Statistical analysis of alleles indicated the following: (1) IL-1A rs1800587 and IL-1B rs1143634: Significant trends were found for the T alleles (p=0.057), which were more frequent in patients with PD and DMAn; (2) IL-1RN rs419598: The allele frequency analysis did not indicate statistically significant differences between the two groups (p=1.000). Conclusion: Combined analysis of genotypes and alleles indicates the following: (1) The frequencies of T alleles and C/T genotypes of the IL-1A rs1800587 and IL-1B rs1143634 genes were statistically significantly higher in patients with PD and DMAn. (2) The frequencies of C/C genotypes of IL-1A rs1800587 and IL-1B rs1143634 genes were statistically significantly higher in PD patients without DMAn. (3) No statistically significant differences were found between the two groups regarding the frequency of genotypes or alleles of the IL-1RN rs419598 gene.