Adipose FoxO1 deficiency induces liver-fat cross-talk for iron metabolism.

(A) adO1KO mice showed upregulation of genes encoding Fe–S binding proteins in gonadal adipose tissue. (B) Non-heme iron content in gonadal adipose tissue (n = 6). (C) qPCR analysis of genes encoding DMT1 (Slc11a2) and TfR1 (Tfrc), n = 8. (D–E) Western blotting (D) and densitometric (E) analyses of iron regulating proteins (n = 6). (F) Serum iron in control (Ctrl) and adO1KO mice (n = 8–12). (G) Non-heme iron contents in the liver (n = 6). (H–I) Western blotting (H) and densitometric (I) analyses of iron-storing, exporting, and importing proteins (n = 6). *, p < 0.05; **, p < 0.01; n.s., not significant.