FoxO1 in adipose browning and energy metabolism.

(A) GO (biological process) analysis of the upregulated genes in gonadal adipose tissue. (B) GO (cellular component) analysis of the upregulated genes in gonadal adipose tissue. (C) Electron microscopic view of gonadal adipose tissue sections from control (Ctrl) and adO1KO mice. (D–E) Western blotting (D) and densitometric (E) analyses of mitochondrial proteins (n = 6). (F) H&E staining of gonadal adipose tissue sections (100x magnification). (G) Real time measurement of oxygen consumption with the TSE system (n = 7). (H) Average oxygen consumption in the dark and light cycles (n = 7). (I) Real time measurement of carbon dioxide production with the TSE system (n = 7). (J) Average carbon dioxide production in the dark and light cycles (n = 7). (K) Respiratory quotient (RQ) measured by the TSE system (n = 7). (L) Real time measurement of energy expenditure (EE) with the TSE system (n = 7). (M) Average energy expenditure (EE) in the dark and light cycles (n = 7). (N) Cold tolerance test (n = 5). *, p < 0.05; **, p < 0.01; ***, p < 0.001.