In-vitro evaluation of Antioxidant and Antimicrobial Activity of Sankarjata (Uraria picta) Leaves Ethanolic Extract

The crude solvents, varying degree of polarity solvents were used to extract Uraria picta. This study used the procedures of a potential antioxidant including DPPH study and a specific act of antimicrobial assay for pathogenic bacteria and fungi to be determined with the Agar well diffusion method and this microdilution method. The antidrug efficacy of Uraria picta extracts was combined with marker antibiotics and tested against MDR bacterial strains to evaluate its potential for resistance modulation. The extracts exhibited potent antioxidant activity with IC50 values adjacent to the standard antioxidant ascorbic acid. Antimicrobial assays demonstrated moderate to strong activity against gram positive; followed by negative bacteria and fungi. Furthermore, the extracts of U. picta increased activity of the standard antibiotics against MDR bacteria, indicating the ability of this plant to reduce drug resistance. This study showcases U. picta as a natural antioxidant and anti-microbial agent and a useful drug for the treatment of many diseases. The proposed mechanism of anti MRD suggests its role as an adjunct in development of strategies for MDR infections. Optimizing the pharmaceutical use of this drug will require further studies on its phytochemical constituents and molecular mechanisms.