Post-Sectioning Verification and Spatial Correlation of 2D Histological Slices with 3D CT-Scans through 3D Printed Phantoms

<p><span>Hard tissue histology is hindered by the fact that the specimen is opaque and therefore the cutting process cannot be targeted to sites of interest. Thus, in the current workflow, relevant structures might be missed or destroyed, and 3D parameters can only hardly be evaluated. Here we present a novel workflow using microCT scans of the specimen prior to cutting, these scans are used for the analysis of 3D features and for directing the sectioning process. Fiducial markers embedded together with the specimen are used to register the obtained histological section into the microCT context. </span></p>