Analytical Method Development for Determination of Salasia and Guduchi in Pharmaceutical Formulation Using

The study focuses on the development and validation of an analytical method for the determination of Salasia and Guduchi in pharmaceutical formulations using advanced chromatographic and spectroscopic techniques. An HPLC chromatographic system was utilized with optimized parameters, including stationary phase, mobile phase composition, flow rate, and detection wavelength. Pure samples of Salasia and Guduchi in pharmaceutical formulations were procured and confirmed using UV-visible wavelength scans and FT-IR spectral analysis. Calibration curves for Salasia and Guduchi demonstrated strong linearity with high R² values. Method development involved optimizing chromatographic conditions to achieve precise and accurate quantitative analysis. Validation studies assessed specificity, linearity, accuracy, precision, and robustness, supported by statistical analysis. System suitability testing ensured optimal chromatographic performance by evaluating retention time, peak symmetry, resolution, and column efficiency. The developed methods were successfully applied to analyze pharmaceutical formulations, demonstrating high reliability. Forced degradation studies under various stress conditions confirmed the stability-indicating capability of the methods. The study provides a comprehensive approach to analytical method development for Salasia and Guduchi, supporting their quality control and pharmaceutical applications. The findings contribute significantly to ensuring the efficacy and stability of these herbal formulations in the pharmaceutical industry.