Anticancer Activity of Ethanolic Extract of Coleus Amboinicus

The present study was designed to evaluate the anticancer activity of ethanolic extract of coleus amboinicus against hepatic cell line (HepG2 cells). In this study we investigate the antioxidant property of the plant. The anticancer activity of the plant extract was evaluated by MTT assay and the cell cytotoxicity was determined. The doses of test used to determine the cell cytotoxicity are: 100µg/ml, 200µg/ml, 300µg/ml, 400µg/ml and 500µg/ml. The test is compared with the standard solution of doxorubicin (~15.98µg/ml). In this study, HepG2 cells were treated with recommended concentrations of the test compound (HB) along with negative and positive controls for an incubation of 24hrs. The experiment results suggested that the test compound (HB) showed dose dependent cytotoxicity, against the HepG2 cell line, with IC50 value of ~ 447.09 µg/ml, after 24hrs of incubation. Coleus amboinicus contain phytochemicals like alkaloids, flavonoids, glycosides phenolic and poly phenolic compounds with potential anti-oxidant properties.