Cutting-Edge Breeding Techniques: CRISPR-Cas9 Applications and Hybridizing Novel Species to Enhance Global Crop Diversity and Agricultural Resilience

In the face of growing agricultural challenges, including climate change, food insecurity, and biodiversity loss, the integration of advanced breeding techniques is essential for the development of superior crop varieties. This thesis examines two transformative methodologies: CRISPR-Cas9 genome editing and interspecies hybridization. CRISPR-Cas9 offers unparalleled precision in modifying traits such as pest resistance, yield enhancement, and improved nutritional content in staple crops.