Microhomology-mediated end-joining Knock-In approaches to delete the allergenic domain of trout parvalbumin beta-1. Preliminary results in F0 animals and feedback

Pages of the laboratory notebook containing PCR gel photographs. Replicon (1077 nt) corresponding to the wild type allele was found in all samples. Replicon (766 nt) demonstrating parvb1 exon 2 deletion was found in only one sample (page 178, lane 8 (sample 81)). Replicon (450 nt) demonstrating parvb1 exon2 swapping was never detected. Pages 167, 169, 173, 174 (lanes 1-10), 178: (Gel photographs of PCR products obtained in experiments aiming to detect exon skipping) Pages 168, 171, 172, and 174 (lanes 12-24): (Gel photographs of PCR products obtained in experiments aiming to detect exon swapping)