ISOLATION AND CHARACTERIZATION OF STIGMASTEROL AND Β- SITOSTEROL FROM ETHYL ACETATE EXCTRACT FROM GNIDIA DAPHNIFOLIA L.F STEMS (THYMELACEAE): A NATIVE PLANT OF MADAGASCAR

Photochemical screening of ethyl acetate extract of Gnidia daphnifolia Lfstems indicated the presence of steroids and triterpene. Columnchromatography of the crude extracts (AcOEt) lead to a number of fractions.Purification of ethyl acetate extract fraction resulted in the isolation ofmixture of two sterols namely Stigmasterol and β-Sitosterol. The isolationcompounds afforded white crystalline powder which was characterized onthe 1D RMN (1H and DEPT) and 2D RMN (HSQC, COSY and HMBC)spectral and compared with their literature data.

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Publication Details

Journal
Zenodo (CERN European Organization for Nuclear Research)
Published
2025-07-19
DOI
https://doi.org/10.5281/zenodo.16165241
Primary Topic
Biological Stains and Phytochemicals
Type
article
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article

ISOLATION AND CHARACTERIZATION OF STIGMASTEROL AND Β- SITOSTEROL FROM ETHYL ACETATE EXCTRACT FROM GNIDIA DAPHNIFOLIA L.F STEMS (THYMELACEAE): A NATIVE PLANT OF MADAGASCAR

Zenodo (CERN European Organization for Nuclear Research)
Biological Stains and Phytochemicals
article

ISOLATION AND CHARACTERIZATION OF STIGMASTEROL AND Β- SITOSTEROL FROM ETHYL ACETATE EXCTRACT FROM GNIDIA DAPHNIFOLIA L.F STEMS (THYMELACEAE): A NATIVE PLANT OF MADAGASCAR

article en

Abstract

Photochemical screening of ethyl acetate extract of Gnidia daphnifolia Lfstems indicated the presence of steroids and triterpene. Columnchromatography of the crude extracts (AcOEt) lead to a number of fractions.Purification of ethyl acetate extract fraction resulted in the isolation ofmixture of two sterols namely Stigmasterol and β-Sitosterol. The isolationcompounds afforded white crystalline powder which was characterized onthe 1D RMN (1H and DEPT) and 2D RMN (HSQC, COSY and HMBC)spectral and compared with their literature data.

Zenodo (CERN European Organization for Nuclear Research)
University of Antananarivo (MG), University of Toliara (MG), Laboratoire de Chimie Organique (FR)
Life in Land
Openalex Percentile: Top 44%
Biological Stains and Phytochemicals
DOIOpen Access PDFOpenAlex
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