Pestalotiopsis clavata Maharachch. & K. D. Hyde

Pestalotiopsis clavata Maharachch. & K. D. Hyde, Fungal. Divers. 56: 108 (2012). Fig. 4 Description. Sexual state not seen. Conidiomata in culture sporodochial, saucer-shaped, scattered or gregarious, superficial to immersed, shining, releasing black conidial masses on the surface. Conidiophores branched, subcylindrical, hyaline to light brown, indistinct, often reduced to conidiogenous cells. Conidiogenous cells cylindrical or ampulliform, hyaline, smooth-walled, solitary to aggregated, (6 –) 7–9 (– 9.5) × (2 –) 2.5–3.5 μm (x ± SD = 7.8 ± 1 × 2.8 ± 0.5 μm). Conidia fusoid, ellipsoid, smooth, slightly constricted at the septa, 4 - septate, (17 –) 19.5–21.5 (– 22.5) × (6 –) 6.5–8 (– 8.5) μm (x ± SD = 20.5 ± 1.2 × 7.4 ± 0.7 μm); apical cell with 2–3 tubular appendages (mostly three); apical appendages arising from an apical crest, unbranched, filiform, bent, (8.5 –) 11–19 (– 25.5) μm (x ± SD = 15 ± 3.8 μm), basal cell with one appendage; basal appendage tubular, centric, unbranched, occasionally swollen at the tip, (3 –) 4–6.5 (– 7) μm (x ± SD = 5.2 ± 1.2 μm) long. Materials examined. CHINA • Zhejiang Province, Wenzhou City, Cangnan County, Dayu Town, 27°22'37"N, 120°36'48"E, on diseased needle of Pinus massoniana, 6 May 2023, Quanchao Wang, Guiyong Cao (cultures ZXD 82, ZXD 99, ZXD 100, ZXD 106); • Zhejiang Province, Wenzhou City, Cangnan County, Zaoxi Town, 27°26'6"N, 120°29'40"E, on diseased needle of Pinus massoniana, 6 May 2023, Quanchao Wang, Guiyong Cao (culture ZXD 124); • Shandong Province, Qingdao City, Jimo District, Tianheng Island Resort, 36°25'2"N, 120°51'37"E, on diseased needle of Pinus thunbergii, 9 August 2023, Quanchao Wang, Guoqing Li, Feifei Liu & Runlei Chang (culture ZXD 565); • Guangdong Province, Jiangmen City, Taishan County, Chixi Town, 21°53'10"N, 112°57'0"E, on diseased needle of Pinus massoniana, 28 August 2023, Quanchao Wang, Guoqing Li, Feifei Liu & Yuhua Liang (culture ZXD 954). Notes. Pestalotiopsis clavata was first reported from Buxus sp. in China (Maharachchikumbura et al. 2012). In this study, seven strains were isolated from P. massoniana and P. thunbergii and clustered together with Pes. clavata in the multi-locus phylogenetic tree (Fig. 3). Compare with the description of ex-type isolate MFLUCC 12-0268, ZXD 954 has shorter appendages (3–7 μm vs. 7–9 μm), while other morphological characteristics are similar. This is the first report of Pes. clavata on the host of P. massoniana and P. thunbergii.