METHOD DEVELOPMENT AND VALIDATION OF GLIMEPIRIDE IN PHARMACEUTICAL DOSAGE FORM BY UV VISIBLE SPECTROPHOTOMETRIC METHOD

Glimiperide is an anti-diabetic drug which is used for the treatment of diabetes. In present work, a simple, sensitive, accurate and economical spectroscopic method has been developed for the estimation of glimepiride in bulk and in pharmaceutical dosage forms. An absorption maximum was found to be at 249 nm with the solvent system of chloroform. The drug follows Beer’s law limits in the range of 5-30 µg/ml with correlation coefficient of 0.999732. Results of the analysis were validated for accuracy, precision, LOD Were found to be satisfactory. The linearity study for Glimepiride was found in concentration range of 5µg-25µg and correlation coefficient (r) was found to be 0.999 and Regression coefficient (r2)0.999 %Recovery studies were carried out and the percentage recovery was found to be in the range of 100.8% - 101%, %RSD of Absorbance for Intraday, Inter day precision was 1 and 0.95, % RSD for Ruggedness was found to be less than 2. LOD and LOQ of calibration curve of drug prepared in 0.1N NaOH were found to be 0.5and 1.5µg/ml, respectively. The proposed method is simple, rapid and suitable for the routine quality control analysis. Key words: Glimiperide, Tablets, UV spectrophotometry.