To An In – Vitro Evaluation of The Anthelmintic Activity of Piper Betle on The Pheritima Posthuma Model

The leaves of piper leaves betle locally known as paan have long been in use in the Indian Indegenous system of medicine of the relief of pain however the underlying molecular mechanism of this effect the have not immunomodulatory effwcts of an ethanolic exctract of the leaves of p. betle were demonstrated in a complete freund adjuvant induced model of arthritis in rats with dexamethasone as the positive control. At non –toxic concentration of PB a dose dependant decrease in extracellular production of nitric oxide in murine peritoneal microphages was measured by the grieas assay and corroborated by flow cytometry using the nitric oxide soecific probe 4,5 –diaminofluorescein -2 diacetate . the decreased generation of reactive nitrogen species was mediated by PB progressively down- regulating transcription of inducible nitric oxide synthase in macrophage , and concomintly causing a dose dependant decrease in macrophage in the expression of synthase in macrophage and concomitantly causing a dose – depedant decrease in the ability of PB to down – regulate T- helper 1 pro – inflamentary and anti-arthiritic activity of PB is attributed to its babillity to down regulate the generation of reactive nitrogen species this meriting further bpharmacological investigation.