Analyzing the Stability of Stored Serum of Different Biochemical Parameters on Different Days

Background: Sample repository is noteworthy, especially in cases of many scientific & medical processes that expedite diagnosis & treatment design. Furthermore, to know the sample integrity which can impact on providing accurate or reproducible data. Materials & Methods: A, total of 11 biochemical parameters were gauged by taking 13 patients’ serum samples at Chikkamagaluru Institute of Medical Sciences, Chikkamagaluru. Each patient’s serum was stowed in an aliquot at -20οC for a month. Initial analysis (T0d) results obtained as fresh samples were compared to subsequent analysis on the 15th (T15d) and 30th (T30d) day of the month. Results: Parameters like glucose, urea, creatinine, uric acid, total bilirubin, AST, ALT, ALP, and albumin expect total protein showed stability at 15 days of analysis. Whereas serum stability was lost for total bilirubin, total protein, direct bilirubin, and ALT during the 30th day of analysis which was statistically significant (p<0.05) Conclusion: Thus, our study shows that parameters like glucose, urea, creatinine, uric acid, albumin, AST, and ALP have shown reproducible values indicating that stability has been maintained when it was stored for up to 30 days. Total protein, bilirubin, direct bilirubin, and ALT are not preferable for analysis, thus affecting the sample integrity.