Phytochemical, FTIR Spectroscopic Analysis and Anti- Bacterial Activity of Poly Herbal Mouth Wash

The aim of the study was to evaluate the microbiological effectiveness of a herbal mouthwash containing the leaves of Psidium guajava (Guava), peels of citrus aurantium (orange), peels of citrus limon, peels of Punica granatum, bark of Cinnamomum zeylanicum (cinnamon against Chlorhexidine mouthwash. Also Quality control parameters like physiochemical, phytochemical and FT-IR analysis (for identification of compounds) were done on developed poly herbal mouthwash formulations. Aqueous extract of Poly herbal formulations was prepared. Standard cultures of, Escherichia coli and Basillus subtilis was used for the study. The antibacterial test used was the agar well disk diffusion method. Marketed formulations of chlorohexidine were compared. In qualitative phytochemical screening, the extracts revealed the presence of primary and secondary metabolites such as carbohydrate, protein, flavonoid, saponin, tannin and sterols. The FT-IR spectral studies of the poly herbal extracts and poly herbal mouthwash preparations have indicated the presence of phytoconstituents compounds. This compound is reported to have wide applications. These results showed that the herbal mouthwash has significant antibacterial activity and the present preparation is able to inhibit bacterial growth in oral cavity. The association of oral microbial load on oral diseases is well established.