Investigations of infected cases in Kirkuk city center through quantitative and qualitative methods of Toxoplasma gondii

Blood samples were taken from 100 blood donations using private laboratories in Kirkuk city. The IHAT, LAT and ELISA tests were used on their blood plasma to see if they had become infected with the Toxoplasma gondii parasite. Researchers learned that, according to direct hemagglutination, the overall infection rate was 44% and by indirect hemagglutination, the rate reached 60%. According to ELISA, 71% of the samples were infected with the virus. After testing methods, it was observed that 44% of samples became infected with a parasite by direct hemagglutination, while 60% became infected by indirect hemagglutination. The infection was found with a 71% positive result by ELISA. The authors observed that the ELISA approach was the most precise method of all in their study, with indirect hemagglutination coming in second and direct hemagglutination ranking third in detecting antibodies. Similarly, T. gondii infection rates were mainly observed in people younger than 35. Our study revealed that the infection rate in people from cities was (75%), while the rate in rural areas was just (25%). The important factors supporting the spread of the parasite are contact with soil, the level of education and keeping cats. ELISA testing revealed that of the infected cases, 75% showed positivity for the IgG antibody compared to 25% for the IgM antibody.

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Publication Details

Journal
Zenodo (CERN European Organization for Nuclear Research)
Published
2025-06-17
DOI
https://doi.org/10.5281/zenodo.15679921
Primary Topic
Toxoplasma gondii Research Studies
Type
article
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article

Investigations of infected cases in Kirkuk city center through quantitative and qualitative methods of Toxoplasma gondii

Zenodo (CERN European Organization for Nuclear Research)
Toxoplasma gondii Research Studies
article

Investigations of infected cases in Kirkuk city center through quantitative and qualitative methods of Toxoplasma gondii

article en

Abstract

Blood samples were taken from 100 blood donations using private laboratories in Kirkuk city. The IHAT, LAT and ELISA tests were used on their blood plasma to see if they had become infected with the Toxoplasma gondii parasite. Researchers learned that, according to direct hemagglutination, the overall infection rate was 44% and by indirect hemagglutination, the rate reached 60%. According to ELISA, 71% of the samples were infected with the virus. After testing methods, it was observed that 44% of samples became infected with a parasite by direct hemagglutination, while 60% became infected by indirect hemagglutination. The infection was found with a 71% positive result by ELISA. The authors observed that the ELISA approach was the most precise method of all in their study, with indirect hemagglutination coming in second and direct hemagglutination ranking third in detecting antibodies. Similarly, T. gondii infection rates were mainly observed in people younger than 35. Our study revealed that the infection rate in people from cities was (75%), while the rate in rural areas was just (25%). The important factors supporting the spread of the parasite are contact with soil, the level of education and keeping cats. ELISA testing revealed that of the infected cases, 75% showed positivity for the IgG antibody compared to 25% for the IgM antibody.

Zenodo (CERN European Organization for Nuclear Research)
University of Tikrit (IQ)
Openalex Percentile: Top 46%
Toxoplasma gondii Research Studies
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Investigations of infected cases in Kirkuk city center through quantitative and qualitative methods of Toxoplasma gondii · Zenodo (CERN European Organization for Nuclear Research) (2025) | TGRS Research Map | TGRS